La lipase de Candida rugosa : caractérisation biochimique

Isolation of Candida rugosa lipase isoforms

The yeast Candida rugosa is a convenient source of lipases for science and industry. Crude preparation of Candida rugosa lipase (CRL) consists of several extracellular lipases. Isoenzyme profile depends on the culture or fermentation conditions. All isoforms are coded by the lip pseudogene family; they are monomers of 534 amino acids and molecular weight of about 60 kDa. They share the same cat...

Inhibition of Candida rugosa lipase by saponins, flavonoids and alkaloids

Citronellyl Butyrate Synthesis in Non-Conventional Media Using Packed-Bed Immobilized Candida Rugosa Lipase Reactor

The synthesis of citronellyl butyrate by direct esterification reaction catalyzed by immobilized lipase from Candida rugosa was studied in a continuous packed bed reactor using n-hexane as organic solvent. Parameters such as residence time, temperature, and pH were examined. The optimum conversion was obtained at a flow rate of 1 ml/min (residence time 8 min), temperature of 50 °C, and pH 7.5. ...

Immobilization of lipase from Candida rugosa on synthetic polymer beads for use in the synthesis of fatty esters ABSTRACT Lipase from Candida rugosa

Lipase from Candida rugosa was immobilized on three different supports, i.e. Amberlite XAD7, poly(methylmethacrylate) (PMMA) and celite. With the conditions tested, maximum adsorption can be achieved after 30 min. The activities of the immobilized lipases were determined by the esterification reaction of oleic acid and butanol. The immobilized lipases were found to be very effective in the este...

Optimization of Candida rugosa lipase immobilization parameters on magnetic silica aerogel using adsorption method

Magnetic silica aerogel in hydrophobic and hydrophilic forms were used as support to immobilize Candida rugosa lipase by adsorption method. Response surface methodology (RSM) was employed to study the effects of the three most important immobilization parameters, namely enzyme/support ratio (0.3-0.5, w/w), immobilization time (60-120 min) and alcohol percentage (20-40, %v/v) on the specific act...